Composite

Part:BBa_K5097011:Design

Designed by: Elber Lopez Hernandez, Kelly Acen, Runyararo Chaora   Group: iGEM24_Oneonta   (2024-09-30)


Acid-sensor, Base-producer pASR-PUT1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2930
    Illegal BamHI site found at 1541
    Illegal BamHI site found at 2843
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1319
    Illegal AgeI site found at 2865
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

SpeA and SpeB were expressed under the same promoter.


Source

pASR(BBa_K1231000) is from the parts repository, and SpeA and SpeB are from E.Coli genome.

References